Technical Support
Click on any of the sections below to view relevant protocols

1

Introduction to Scaffold Formats

Topics covered in this section:

  • Experimental Design
  • Choosing cells media & supplements
  • Planning end-point analysis & length of study
2

Scaffold Set-Up and Cell Seeding

Topics covered in this section:

  • Activation of Alvetex®Scaffold
  • Cell Seeding
  • Use of Coating agents with Alvetex®Scaffold
  • Recommended seeding density
3

Culture Maintenance and Monitoring of Cell Growth

Topics covered in this section:

  • Monitor / optimise cell attachment/growth
  • Visualisation throughout experimental time-course
  • Carrying out required media changes
4

End-point Analysis of your 3D Cell Culture

Topics covered in this section:

  • Visualisation of cells 1: Histology
  • Visualisation of cells 2: Immunofluorescence, Confocal microscopy
  • Visualisation of cells 3: SEM and TEM
  • End-point analysis of 3D cell cultures
  • Cell Retrieval
  • White paper on imaging techniques.
    2
  • Successful Confocal Microscopy of 3D Cell Cultures
  • Quick Start Protocol
  • Quick Start Alvetex®Scaffold 96
  • Compiled Protocol Document
  • Alvetex® 96 well
  • Instructional Videos
    2
  • Images
    5
  • Videos
    1
  • Coating
    5
  • Cell Specific Protocols
    15
  • Transfection
    1
  • Cell Visualisation Protocols
    3
  • Metabolic Assay Protocols
    5
  • Images
    5
  • Genomics & Proteomics
    3
  • Advanced Microscopy
    2
  • Histology & Immunocytochemistry
    5
  • Cell Retrieval
    1
  • images
    4
  • FAQs
  • Contact Us

Microscopy Protocols

Cell Retrieval

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